eng
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
2018-08-01
2
2
1
8
10.21608/jcbr.2018.37763
37763
Original Article
Administration of olive oil optimizes acetic acid induced colitis in CD1 mice
Dina Ali
dinaabdelaziz123@yahoo.com
1
Mohamed Salem
cecr@unv.tanta.edu.eg
2
Ehab Tousson
3
Department of Zoology, Faculty of Science, Tanta University, Egypt.
Immunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Egypt. Center of Excellence in Cancer Research, Tanta Teaching Hospitals.
Zoology Department, Faculty of science, Tanta University, Egypt.
Colitis is a chronic disease that affects individuals at different ages and affects the quality of their life. So far, there are three different experimental models for colitis. However, animals in these models die quickly duo to the associates toxicity. The aim of this study is to optimize acetic acid induced acute colitis mouse model with olive oil co-administration. Male albino CD1 mice (n= 91) were randomly divided into 13 groups (n= 7/group). To induce colitis, mice were administrated with different volumes (100 or 200 µl) and concentrations (1%, 2% and 4%) of acetic acid by intrarectal (IR) injection either for three consecutive days or every other day for a total of 6 days. Olive oil (1 µL) was co-injected with acetic acid. One day later, mice were sacrificed and colon tissues were fixed in 10% formalin for histological examinations. The induction of colitis by acetic acid was dependent on both the volume and the concentration of acetic acid. Interestingly, co-administration of olive oil with different volumes and concentrations of acetic acid, although did not alter the severity of inflammation, it increased the animal survival rate and decreased the associated toxicity. Injection of co-administration of olive oil with acetic acid is a new modification to enhance the application of this model.
https://jcbr.journals.ekb.eg/article_37763_6987864b51bf97435b6f86dbf322b6fb.pdf
acetic acid
colitis
Colon
model
eng
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
2018-08-01
2
2
9
13
54421
Original Article
High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients
Mohamed Salem
mohamed.labib@science.tanta.edu.eg
1
Hanan Elokeily
nona_25689@yahoo.com
2
Ibrahim ElShourbgy
3
Nadia Elwan
nad.dec1983@gmail.com
4
Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
Department of Tropical Medicine, Faculty of Medicine, Tanta University, Tanta, Egypt
Background: Regulatory T cells (Tregs), possess a suppression function leading to T cells exhaustion and tumor progression through serious of signaling pathways of inhibitory receptors under pathological conditions in particular cancer including hepatocellular carcinoma. Conventional helper CD4+ T cells can be converted into regulatory T cells that may suppress anti HCC immunity through different mechanisms including expression of PD-1. PD-1 inhibitory checkpoint and its ligand PDL-1 emerge immune escape through interaction between T cell and tumor microenvironment. Aim: The aim of this study is to analyze the expression level of the regulatory checkpoint (PD-1) on T cells in HCC patients. Materials and methods: The numbers of conventional and regulatory T cells and their expression of checkpoint receptor PD-1 were analyzed in the peripheral blood of HCC patients (n=20) as well as healthy control volunteers (n=15) using multi-parametric flow cytometry after staining with anti-CD4, anti-CD25, anti-CD127 for (T cells) and anti PD-1 (CD279). Results: As compared to healthy control volunteers HCC patients showed high relative numbers of Tregs expressing CD4+CD25+CD127- and decrease relative number of conventional CD4+ defines as CD4+CD25-CD127-. These cells showed high expression by increasing level of PD-1 where CD4+ showed high expression than Tregs. Similar cells number of PD-1 expression where observed at the level of absolute numbers. Conclusion: HCC patients before treatment express high level of the checkpoint molecule PD-1 opening the door for further investigations.
https://jcbr.journals.ekb.eg/article_54421_d41d8cd98f00b204e9800998ecf8427e.pdf
Cancer
CD4
Checkpoint
Hepatocellular carcinoma
Immune
PD-1
Regulatory T cells
eng
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
2018-07-30
2
2
14
20
54431
Original Article
Protective activities of Nigella sativa and its active gradient thymoquinone on the carcinogenic effects of N-butyl-N-(4-hydroxybutyl)nitrosamine on Wistar rats
Elsayed Salim
elsalem_777@yahoo.com
1
Areeg Khalifa
areegkh90@gmail.com
2
Zoology Department, Faculty of Science, Tanta University, Egypt
Tanta University, Faculty of Science, Department of Zoology
Background: Carcinogens become a serious environmental challenge. Aim: The main aim of this study was to assess the alterations in some biochemical parameters in male Wistar rats after. Materials and Methods: Experimental groups were administered with 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) followed by 5% Sodium ascorbate (Na-As), then they were post-treated with Nigella sativa (N. sativa) by an inter-gastric luminal gavage or with thymoquinone (TQ) by inter-peritoneal injections. Blood samples were collected after a 32 weeks’ experiment. Results: TQ seemed to have a powerful inhibitory effect on total cholesterol and triacylglycerol levels. While levels of A/G ratio, ALT and AST were highly decreased by both of N. sativa and TQ. Conclusion: These findings may rely on the lipid-modifying and hepatoprotective effects of N. sativa crude oil and its main constituent TQ on mammals. Thus, further investigations are required.
https://jcbr.journals.ekb.eg/article_54431_33a608487530a994ed8c6df52f81d6e3.pdf
Keywords: N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Sodium ascorbate (Na-As)
Nigella sativa (N. sativa)
Thymoquinone (TQ)