The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
2
1
2018
04
01
In vitro Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines
1
10
EN
Reham
N
Mohasseb
Zoology Department, Faculty of Science, Tanta University, Egypt
rery.mohasseb@hotmail.com
Mohamed
Salem
0000-0001-9454-6327
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Center of Excellence in Cancer Research, Tanta Teaching Hospitals
cecr@unv.tanta.edu.eg
Reda
M.
Gaafar
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
Mohamed
A
Abd-Elbaseer
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
10.21608/jcbr.2019.37759
The extracts of the medicinal plants <em>Achillea santolina</em> and <em>Raphanus sativus</em> have been reported to show anti-cancer effects <em>in vitro</em>. However, the cellular and molecular mechanism of these effects are not clear yet. To compare the apoptotic effects of these plant extracts on different cancer cell lines <em>in vitro</em>. The phenolic, flavonoids and antioxidant activity were determined in the crude extracts. Then, Caco2 (colon adenocarcinoma), HepG2 (hepatic carcinoma), MCF7 (breast cancer) and the normal WISH (amniotic cell line) were treated <em>in vitro</em> with different concentrations of crude extracts for 72 hours. The half maximal inhibitory concentration (IC<sub>50</sub>) were detected by MTT assay, while cell cycle and apoptosis were assessed by flow cytometry. The methanolic extract of <em>R. sativus</em> seeds (cultivar: Balady) showed higher phenolic content (791.98 mg/d.wt) and higher antioxidant activity (93%) than those of the ethanolic extract of <em>A. santolina</em> (340.23 mg/d.wt) and (72.72%), respectively. <em>R. sativus</em> methanolic extract showed lower flavonoids contents (1.025 mg/g d.wt) than <em>A. santolina</em> ethanolic extract (24.66 mg/g d.wt). Treatment of Caco2, HepG2, MCF7 and WISH cell lines with <em>A. santolina</em> extract showed IC<sub>50</sub> of 17.67 µg/ml, 15.12 µg/ml, 42.19 µg/ml and 50.99 µg/ml, respectively. While treatment of the same cell line with <em>R. sativus</em> showed IC<sub>50</sub> of 40.77 µg/ml, 27.42 µg/ml, 54.16 µg/ml and 86.37 µg/ml respectively. <em>A. santolina</em> and <em>R. sativus</em> extracts induced similar cell cycle arrest in Caco2 at G1 phase by 42.4%. This study indicates that <em>A. santolina</em> has a potent anticancer activity against the selected cancer cell lines.
Achillea santolina,Raphanus sativus,cell lines,Cytotoxicity,Cell cycle,apoptosis
https://jcbr.journals.ekb.eg/article_37759.html
https://jcbr.journals.ekb.eg/article_37759_5dff44824a3b32fcdaaf57266049da85.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
2
1
2018
04
01
Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C)
11
18
EN
Mohamed
Salem
0000-0001-9454-6327
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
cecr@unv.tanta.edu.eg
Essam
M.
Ibrahim
Histology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Hassan
El-Bate
Tropical Medicine and Infectious Diseases Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Abdel-Rahman
N.
Zekri
Cancer biology Unit, National Cancer Institute, Cairo University, Cairo, Egypt
Hala
A.
Abdelhady
Tanta Cancer Center, Tanta, Egypt
10.21608/jcbr.2019.34740
Chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) resulted in dysfunction of the immune response, in particular, dendritic cells (DCs). Stimulation and reactivation of DCs could restore the immune responses in this disease. Our previous study revealed that a synthetic double-stranded RNA (polyI:C) recognized by toll-like receptor 3 (TLR3) induced a potent innate immune response and had an impact on DCs both ex vivo and in vivo. The current study was aimed to generate and maturate DCs from peripheral blood mononuclear cells (PBMCs) of healthy controls and patients with HCV and HCC. Fresh peripheral blood (PB) was collected from healthy controls, patients with chronic HCV or patients with hepatocellular carcinoma (HCC). PBMCs were isolated and cultured for 6 days in RPMI-1640 supplemented with GM-CSF and rhIL-4 (10 ng/mL each). Poly(I:C) was added to the culture on day 6 and the cells were harvested on day 7. Phenotypic analysis of DCs and their maturation markers were assessed using flow cytometry. DCs were generated from adherent PBMCs of healthy donors or patients with HCV or HCC. Interestingly, the addition of poly(I:C) induced expansion and maturation of DCs as evidenced by the expression of HLA-DR and CD11bCD11c surface molecules on the DCs generated from all groups. In conclusion, DCs can be ex vivo generated from control or patients with HCV or HCC in response to external stimulation. These results may be a promising tool for a therapeutic vaccine against HCV infection
Poly(I:C),Dendritic cells,HCV,HCC
https://jcbr.journals.ekb.eg/article_34740.html
https://jcbr.journals.ekb.eg/article_34740_6aa3222e4bfd0a862d999b642bd57130.pdf