The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
2
2019
08
01
Assessing mother’s antibiotics information by comparing internet and healthcare professionals as sources of informatics
1
7
EN
Huda
Kutrani
Health Informatics Department, Faculty of Public Health, University of Benghazi, Libya
huda.kutrani@uob.edu.ly
Antisar
A
Atiyah
2Health Education Department, Faculty of Public Health, University of Benghazi, Libya
Hend
M
Adam
Health Education Department, Faculty of Public Health, University of Benghazi, Libya
Haloom
A
Elhashmi
Pediatric Department, Faculty of Medicine, University of Benghazi, Libya
10.21608/jcbr.2019.37773
Parents especially mothers view the internet as a source to obtain information about their children’s health care. This study was designed to assess mother’s antibiotics information by comparing the Internet and healthcare professionals as sources of informatics. A cross-sectional descriptive study was performed in the outpatient pediatrics clinics at the Children Hospital and Benghazi Medical Centre, from February 2018 to June 2018. The sample size was 308 mothers attending the outpatient pediatrics clinic during the period of data collection. Knowledge/beliefs, behaviors, adherence, and awareness of antibiotic resistance were determined through the analysis of data obtained from the questionnaires. Eighty-two percent of mothers had a poor level of knowledge and beliefs regarding antibiotic use; however, about half of the mothers had good behaviors toward the antibiotic and a good level of awareness of antibiotics resistance. Despite this poor knowledge, 61.7% of the mothers had an overall good Adherence to antibiotic use. Furthermore, half of the mothers obtained their information about the antibiotic from the internet. A good knowledge level and good awareness of antibiotics resistance levels were attained from the Internet source. Meanwhile a good behavior and good adherence regarding antibiotic usage were gained from a healthcare professional. In conclusion, mother’s good information showed a significant difference existed between the Internet and the healthcare professional sources. Therefore, this study recommends that healthcare professionals should educate mothers concerning online information. Moreover, there is a need to improve mother’s knowledge about antibiotics uses.
antibiotics information on Internet,misuse,Mothers,resistance
https://jcbr.journals.ekb.eg/article_37773.html
https://jcbr.journals.ekb.eg/article_37773_7f8cad0ac522114c8e346ca23ecc67ae.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
2
2019
08
01
Biochemical Alteration and Immunohistochemical Characterization of Lymphoid Cells Involved in Type 2 Diabetes Mellitus
8
19
EN
Nahla
H
El-shaer
Zoology Department, Faculty of Science, Zagazig University, Egypt
nahlaelshaer@yahoo.com
10.21608/jcbr.2019.37782
<strong>Background</strong>: T cells are crucial for the inflammation development of the metabolic disorder and insulin resistance and the cytokines of T cells are considered as an excellent evaluating tool for the immune system activity in many diseases. As cytokine profile associates with the development of type-2 diabetes mellitus (T2-DM) is well understood the link between this disease and the involved immune cells still unclear. as such, it was hypothesized that the Th1 pathway is disrupted in the pathogenesis of type 2DM. <strong>Aim</strong>: The main aim of this study was to evaluate IL-12, which induces the Th1 pathway, and IL-2 which mediates the Th1 function. <strong>Materials and Methods</strong>: Paraffin-embedded sections of peripheral blood lymphocytes with T2-DM patients were stained with antibodies against CD4<sup>+</sup> and CD8<sup>+</sup> T cells. Computerized image analysis was used to calculate areas of stained lymphocytes taken from diabetic and control patients. Clinical data and blood samples were collected from Eighty-one (37.6±13.9 years old) diabetic Egyptian patients and 103 (40.5±12.7 years old) of normal control volunteer to evaluate the level of serum interleukin IL-2 and IL-12 in their blood by using ELISA technique. Routine laboratory analysis, including fasting blood glucose, CRP, lipid profile, fasting insulin, and CBC for each patient were measured. <strong>Results</strong>: Immunohistochmical results in 2DM showed an increase in the numbers of CD8<sup>+</sup> cells with a decrease in the numbers of CD4<sup>+</sup> cells displayed. Diabetic patients showed decreased and increased values of IL-2 and IL-12, respectively as compared to control values. <strong>Conclusion</strong>: This data indicates the importance of these two cytokines in the pathogenesis of type-2 Diabetes mellitus.
CD4,CD8,Immunohistochemical,IL-12,diabetes,T cells,Type 2
https://jcbr.journals.ekb.eg/article_37782.html
https://jcbr.journals.ekb.eg/article_37782_9f0a1e570b8e4091e963434fd428a436.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
2
2019
08
30
Hematological and toxicological parameters after metformin treatment during pancreatic carcinogenesis
20
26
EN
Mohamed A.
M.
Hegazi
https://orcid.org/my
Zoology Department, Faculty of Science, Tanta University, Tanta 31527-Egypt.
m_a_m_hegazi@hotmail.com
Elsayed
Salem
Zoology Department, Faculty of Science, Tanta University, Egypt
Ahmed
Nassem
Zoology Department, Faculty of Science, Tanta University, Egypt
ahmednaseem85@gmail.com
Pancreatic cancer is the fourth-highest cancer killer among both men and women worldwide. In Egypt, pancreatic cancer incidence and mortality rates has been increasing in the last few years due to unknown reasons. The biguanides metformin is the most widely used first-line therapy for type 2 diabetes (T2D). Numerous observational studies have supported a protective role for metformin against a variety of cancer types as breast, liver and prostate cancer. This study aimed to investigate the anti-tumor effects of metformin treatment against pancreatic cancer induced in Syrian golden hamsters.by subcutaneous injection of A/-nitrosobis (2-oxopropyl) amine (BOP). Gemcitabine was used as a conventional drug used for treatment of pancreatic cancer. The results show that metformin has no toxic effect against chemically induced pancreatic carcinogenesis of hamsters caused by BOP induction.
Metformin,Hamsters,Pancreatic cancer,N-Nitrosobis (2-oxopropyl) amine
https://jcbr.journals.ekb.eg/article_54440.html
https://jcbr.journals.ekb.eg/article_54440_700047f1a14bdb5f4d316dfe3f4de075.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
2
2019
11
30
In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles
27
38
EN
Yousry
E
El-Bolkiny
0000-0003-4001-6034
Department of Zoology, Faculty of Science, Tanta University, Egypt
bolkiny61@yahoo.com
El-Refaie
Kenawy
Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
Ibrahim
Al-Shourbagy
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Mohmed
Basyony
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Reda
Salama
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Abdelhalim
Alkhazendar
0000-0001-9427-5985
Zoology Department, Faculty of Science, Tanta University
abdelhalimalkhazendar@gmail.com
Biologically active agents show several biomedical applications. Non-chitosan loaded diethyldithiocarbamate (DDC) was previously found to have anti-schistosomal activity. The present work aimed to prepare DDC-Chitosan-Na-nanoparticles (DDC-Chitosan-Na-NPs), and to investigate it’s in vitro anti-schistosomal activity with slow release approach and as well as its mortality % against both concentrations and exposure time. NPs were prepared by suspension cross linking method, in which 0.16 g chitosan were dissolved in 16 ml 2% acetic acid solution (ratio 1% wt/v.). Then after, 0.1g DDC-Na salt was added to the chitosan solution and 3.2 ml of glutaraldehyde as a cross linking agent was added into the mixture dropwise. NPs were washed several times with ethanol 70%, dried under vacuum, prepared for chemical characterization and biological assessment. Our data showed that the slow release of DDC-chitosan-NPs resulted in mortality effects on Schistosomal worms in vitro when applied with small dose for longer time. These data compared to those of prazoquantil (PZQ) as a reference drug showed that administration of DDC loaded with chitosan-NPs was more effective on the mortality of Schistosoma worms. In conclusion, DDC-Chitosan-NPs have lethal effects on S. mansoni worms in vitro by a unique mode of action in a way of concentration and exposure time dependent. Also we recommend that the development of DDC as a local, cheaper and more effective agent against S. mansoni worms.
Metformin,Hamsters,Pancreatic cancer,N-Nitrosobis (2-oxopropyl) amine
https://jcbr.journals.ekb.eg/article_63072.html
https://jcbr.journals.ekb.eg/article_63072_4ffd8736b9b173a4282411d8ec8867b0.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
2
2019
08
01
Ameliorating effects of some trematode antigens on arthritis induced in Wistar rats
39
49
EN
Mohamed
Salem
0000-0001-9454-6327
Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt
mohamed.labib@science.tanta.edu.eg
Ahlam
Aboshafey
Zoology Department, Faculty of Science, Tanta University, Egypt
ahlam.aboushafei@science.tanta.edu.eg
Lamia
Bakr
Zoology Department, Faculty of Science, Tanta University, Egypt
lamiabakr@yahoo.com
Shimaa
Mohamed
Zoology Department, Faculty of Science, Tanta University, Egypt
shimaa.mohamed2255@yahoo.com
10.21608/jcbr.2020.26221.1016
<strong>Introduction</strong>: Rheumatoid Arthritis (RA) is an autoimmune disease mediated by T helper 1 immune cells (Th1). <strong>Aim</strong>: Given that parasitic antigens are capable of polarizing the immune response towards T helper 2 immune cells (Th2), this study aimed to assess the potential effect of <em>Schistosoma mansoni </em>soluble egg antigen (Sm)<em> </em>and <em>fasciola hepatica</em> adult worm antigen (Fh) which are known to induce Th2 type response. <strong>Materials and Methods:</strong> RA was induced in female Wistar rats by intradermal injection (i.d) of 0.1ml complete Freund’s adjuvant (CFA). After 12 days of CFA injection, rats were treated with i.d injection of 0.1 mL saline, 0.2 mg/mL methotrexate (MXT via intraperitoneal injection, i.p)) once a week four 3 consecutive weeks as a reference drug, 100 μg Sm (i.p) or (i.d), 100 μg Fh (i.p) and (i.d). Rats were treated with antigens daily for two weeks. <strong>Results</strong>: Compared to control RA rats, treatment with Sm orFh antigens resulted in significant amelioration in the symptoms of the disease, including paw thickness and deformity measured by X-ray. These effects were further confirmed by significant alteration in histopathological changes in the hind paw. Analysis of Th1 and Th2 cytokines axis revealed high serum levels of IL-6 and TNF-a and lower serum levels of IL-4 and IL-10. Analysis of liver function showed that treatment with Sm or Fh antigens resulted in significant amelioration in hepatic enzymes including ALT, AST, and ALP. Treatment with Fh orSm also induced significant amelioration in the increased number of white blood cells and the absolute numbers of neutrophils. <strong>Conclusion</strong>: Overall, our data indicate that Sm possesses higher anti-arthritic effect than Fh, suggesting that trematode antigens can be used as a potential therapeutic approach to ameliorate arthritis.
Fasciola hepatica, Methotrexate, Rheumatoid Arthritis,Schistosoma mansoni
https://jcbr.journals.ekb.eg/article_81295.html
https://jcbr.journals.ekb.eg/article_81295_930e5d0fdfacd78d0a215b7978ed02fd.pdf