The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
3
2019
12
01
High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients
2
7
EN
Mohamed
Labib
Salem
0000-0001-9454-6327
Professor of Immunology
Immunology & Biotechnology Division
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
cecr@unv.tanta.edu.eg
Nadia
M.
Elwan
Tropical Medicine and Infectious Disease Department, Faculty of Medicine, Tanta University, Egypt.
Ibrahim
ElShourbgy
Department of Zoology, Faculty of Science, Tanta University, Tanta.
Hanan
A.
Elokeil
Center of Excellence of Cancer Research, Tanta University Educational Hospital
nona_25689@yahoo.com
<strong>Background</strong>: Regulatory T cells (Tregs), possess a suppression function leading to T cells exhaustion and tumor progression through serious of signaling pathways of inhibitory receptors under pathological conditions in particular cancer including hepatocellular carcinoma. Conventional helper CD4<sup>+ </sup>T cells can be converted into regulatory T cells that may suppress anti HCC immunity through different mechanisms including expression of PD-1. PD-1 inhibitory checkpoint and its ligand PDL-1 emerge immune escape through interaction between T cell and tumor microenvironment. <strong>Aim</strong>: The aim of this study is to analyze the expression level of the regulatory checkpoint (PD-1) on T cells in HCC patients. <strong>Materials and methods</strong>: The numbers of conventional and regulatory T cells and their expression of checkpoint receptor PD-1 were analyzed in the peripheral blood of HCC patients (n=20) as well as healthy control volunteers (n=15) using multi-parametric flow cytometry after staining with anti-CD4, anti-CD25, anti-CD127 for (T cells) and anti PD-1 (CD279). <strong>Results</strong>: As compared to healthy control volunteers HCC patients showed high relative numbers of Tregs expressing CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>-</sup> and decrease relative number of conventional CD4<sup>+</sup> defines as CD4<sup>+</sup>CD25<sup>-</sup>CD127<sup>-</sup>. These cells showed high expression by increasing level of PD-1 where CD4<sup>+</sup> showed high expression than Tregs. Similar cells number of PD-1 expression where observed at the level of absolute numbers. <strong>Conclusion: </strong>HCC patients before treatment express high level of the checkpoint molecule PD-1 opening the door for further investigations.
Cancer,CD4,Checkpoint,Hepatocellular carcinoma,Immune,PD-1,Regulatory T cells
https://jcbr.journals.ekb.eg/article_63386.html
https://jcbr.journals.ekb.eg/article_63386_d41d8cd98f00b204e9800998ecf8427e.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
3
2019
12
17
Comparing the possible anti-tumor effects of synthetic chalcones and metal complexes in a tumor mouse model
17
27
EN
Asmaa
Ahmed
El Gamal
Department of Zoology, Faculty of Science, Tanta University, Egypt
asmaa_elgamal90@yahoo.com
Mohamed
Nasef
Immunology and Biotechnology Unit, Zoology Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt
nassefssd@science.tanta.edu.eg
Mohamed
Gaber
Department of Chemistry, Faculty of Science, Tanta University, Egypt
abuelazm@yahoo.com
Mohamed
Salem
0000-0001-9454-6327
Immunology and Biotechnology Unit,
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
mohamed.labib@science.tanta.edu.eg
10.21608/jcbr.2019.65449
<strong>Introduction</strong>: Traditional treatment of cancer by using chemotherapy not only kills cancer cells but also, normal cells and failed in preventing the spread of the tumor. Chalcone have several biological activities, such as antioxidant, antibacterial, antifungal, anti-inflammatory, and anti-tumor. <strong>Aim</strong>: To compare the antitumor effects of chalcones in Ehrlich ascetic carcinoma (EAC) bearing mice. <strong>Materials and Method</strong>: Female Swiss albino mice were randomly divided into 6 groups as the following; Group 1 were served as a negative control, group 2 were inoculated with 2.5×10<sup>5</sup> (EAC) cells, group 3 were inoculated with 2.5×10<sup>5</sup> EAC cells and then treated with cisplatin (2 mg/kg), group 4 were inoculated with 2.5×10<sup>5</sup> EAC cells and then treated with chalcone 1 (0.3 ml gm/L), group 5 were inoculated with 2.5×10<sup>5</sup> EAC cells and then treated with chalcone II (0.3 ml gm/L). After 7 days, all groups of mice were sacrificed to measure the number and cell cycle of tumor cells as well as the number and activation of CD8<sup>+</sup> T cells. <strong>Results</strong>: Treatment of tumor-bearing mice with chalcone I, but not chalcone II induced decreases in the total number of live cells when compared to control tumor-bearing mice, coinciding with significant increases in G0, G1, S and G2 phases of EAC cells which were comparable to the effects of cisplatin. As compared to control tumor-bearing mice, tumor cells harvested from mice treated with chalcone I showed significant increases in the numbers of activated CD8<sup>+</sup> T cells. <strong>Conclusion</strong>: Chalcone I possesses anti-tumor effects by inducing tumor cells arrest and activation of T cells.
Chemotherapy,Cisplatin,Chalcone,Cell cycle, CD8+ T cells, Ehrlich ascites carcinoma, Metal complex,
https://jcbr.journals.ekb.eg/article_65449.html
https://jcbr.journals.ekb.eg/article_65449_d41d8cd98f00b204e9800998ecf8427e.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
3
2019
12
17
Effect of Balanitoside and Gemcitabine on serum biochemistry during mouse lung carcinogenesis
28
33
EN
Elsayed
Salim
Zoology Department, Research Lab. of Molecular Carcinogenesis
elsalem_777@yahoo.com
Fouad
Abou-Zaid
Zoology Department, Research Lab. of Molecular Carcinogenesis
fouadabozaid@yahoo.com
Abeer
Khamis
Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
lamarbasel2013@yahoo.com
Sara
S
Abou-Eisha
Zoology Department, Research Lab. of Molecular Carcinogenesis
s.s.hussein2@gmail.com
<strong>Background</strong>: Gemcitabine is utilized as a standard anti malignancy chemotherapy. Its use, however, is limited due to the associated serious side effects. <strong>Aim</strong>: The aim of the present study is to study the impact of balanitoside, extracted from <em>Balanites aegyptiaca</em> fruits on mouse serum biochemistry during chemically-induced lung cancer. Methods: Mice were treated with the carcinogen (Ure+ BHT) to induce lung carcinogenesis and then left without treatment, treated gemcitabine, or both gemcitabine and balanitoside. <strong>Results</strong>: Our results reveal that elevated serum levels of alanine and aspartate transaminases caused by Ure+BHT injection were inhibited in mice treated with the ethanolic extract of <em>B. aegyptiaca</em>, indicating to its hepatoprotective actions either alone combined with gemcitabine. Also, the doses of <em>B. aegyptiaca</em> extract used here enhanced the renal function parameters as it improved the changes induced by Ure+BHT occurred in serum creatinine and uric acid levels. Moreover, the present data showed increased serum cholesterol, triglyceride, and low-density lipoprotein (LDL) levels and a decrease in high-density lipoprotein (HDL) levels after carcinogen-treatment when compared with the control normal group. These changes were ameliorated after treatment with <em>B. aegyptiaca</em> either alone or combined with gemcitabine. <strong>Conclusion</strong>: <em>B. aegyptiaca</em> extract has significant modulatory effects and chemo-preventive properties against biochemical changes during lung carcinogenesis, indicating to its prophylactic anti-toxic effect.
Balanitoside,Gemcitabine,mouse lung cancer,liver function,Kidney function,Lipid profile
https://jcbr.journals.ekb.eg/article_65450.html
https://jcbr.journals.ekb.eg/article_65450_d41d8cd98f00b204e9800998ecf8427e.pdf
The Egyptian Association for Cancer Research (EACR)
International Journal of Cancer and Biomedical Research
2682-261X
2682-2628
3
3
2019
12
26
Effect of Moringa oleifera on antioxidant enzymes and oxidative stress induced by aluminium exposure in male albino rat testes
34
41
EN
Hala
Abdelazem
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
kareemayad1993@gmail.com
This study summarizes the effect of Moringa oleifera leaves extract on oxidative stress-induced from exposure to aluminium (aluminium chloride, AlCl₃) on testes of male albino rats. Fifteen male albino rats (250.0±10.0) g were divided into five groups, each group of 10 rats. Group one received sterile water orally as a control, group two received AlCl₃ (50mg/kg/day), group three received M. oleifera (300mg/kg/day), group four received AlCl3 (50mg/kg/day) for four weeks then M. oleifera (300mg/kg/day) for four weeks and last group five received AlCl₃ and M. oleifera for eight weeks. The results showed significant changes in testes parameters where there was a decrease in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione -s-transferase (GST) and glutathione peroxidase (GPx) associated with increase in the activity of xanthine oxidase (XO) and malondialdehyde (MDA) levels in aluminium treated groups. Also, there was an improvement in the activity of the enzymes (SOD, CAT, GST, and GPx) associated with a decrease in the activity of XO and MDA in groups received M. oleifera. These results proved that M. oleifera has an ameliorative effect on the activities of antioxidant enzymes and oxidative stress-induced from exposure to aluminium in male albino rats.
https://jcbr.journals.ekb.eg/article_66836.html
https://jcbr.journals.ekb.eg/article_66836_b469db1d7389e5f769b0c11fc07f98e7.pdf