The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X2220180801Administration of olive oil optimizes acetic acid induced colitis in CD1 mice183776310.21608/jcbr.2018.37763ENDina Abd El AzizAliDepartment of Zoology, Faculty of Science, Tanta University, Egypt.MohamedSalemImmunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Egypt.
Center of Excellence in Cancer Research, Tanta Teaching Hospitals.0000-0001-9454-6327Ehab MToussonZoology Department, Faculty of science, Tanta University, Egypt.Journal Article20180424Colitis is a chronic disease that affects individuals at different ages and affects the quality of their life. So far, there are three different experimental models for colitis. However, animals in these models die quickly duo to the associates toxicity. The aim of this study is to optimize acetic acid induced acute colitis mouse model with olive oil co-administration. Male albino CD1 mice (n= 91) were randomly divided into 13 groups (n= 7/group). To induce colitis, mice were administrated with different volumes (100 or 200 µl) and concentrations (1%, 2% and 4%) of acetic acid by intrarectal (IR) injection either for three consecutive days or every other day for a total of 6 days. Olive oil (1 µL) was co-injected with acetic acid. One day later, mice were sacrificed and colon tissues were fixed in 10% formalin for histological examinations. The induction of colitis by acetic acid was dependent on both the volume and the concentration of acetic acid. Interestingly, co-administration of olive oil with different volumes and concentrations of acetic acid, although did not alter the severity of inflammation, it increased the animal survival rate and decreased the associated toxicity. Injection of co-administration of olive oil with acetic acid is a new modification to enhance the application of this model.The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X2220180801High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients91354421ENMohamedSalemImmunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt0000-0001-9454-6327Hanan Abd ElGhanyElokeilyZoology Department, Faculty of Science, Tanta University, Tanta, EgyptIbrahimElShourbgyZoology Department, Faculty of Science, Tanta University, Tanta, EgyptNadiaElwanDepartment of Tropical Medicine, Faculty of Medicine, Tanta University, Tanta, EgyptJournal Article20180318<strong>Background</strong>: Regulatory T cells (Tregs), possess a suppression function leading to T cells exhaustion and tumor progression through serious of signaling pathways of inhibitory receptors under pathological conditions in particular cancer including hepatocellular carcinoma. Conventional helper CD4<sup>+ </sup>T cells can be converted into regulatory T cells that may suppress anti HCC immunity through different mechanisms including expression of PD-1. PD-1 inhibitory checkpoint and its ligand PDL-1 emerge immune escape through interaction between T cell and tumor microenvironment. <strong>Aim</strong>: The aim of this study is to analyze the expression level of the regulatory checkpoint (PD-1) on T cells in HCC patients. <strong>Materials and methods</strong>: The numbers of conventional and regulatory T cells and their expression of checkpoint receptor PD-1 were analyzed in the peripheral blood of HCC patients (n=20) as well as healthy control volunteers (n=15) using multi-parametric flow cytometry after staining with anti-CD4, anti-CD25, anti-CD127 for (T cells) and anti PD-1 (CD279). <strong>Results</strong>: As compared to healthy control volunteers HCC patients showed high relative numbers of Tregs expressing CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>-</sup> and decrease relative number of conventional CD4<sup>+</sup> defines as CD4<sup>+</sup>CD25<sup>-</sup>CD127<sup>-</sup>. These cells showed high expression by increasing level of PD-1 where CD4<sup>+</sup> showed high expression than Tregs. Similar cells number of PD-1 expression where observed at the level of absolute numbers. <strong>Conclusion: </strong>HCC patients before treatment express high level of the checkpoint molecule PD-1 opening the door for further investigations.The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X2220180730Protective activities of Nigella sativa and its active gradient thymoquinone on the carcinogenic effects of N-butyl-N-(4-hydroxybutyl)nitrosamine on Wistar rats142054431ENElsayed I.SalimZoology Department, Faculty of Science, Tanta University, EgyptAreegKhalifaTanta University, Faculty of Science, Department of ZoologyJournal Article20180424<strong>Background</strong>: Carcinogens become a serious environmental challenge. <strong>Aim</strong>: The main aim of this study was to assess the alterations in some biochemical parameters in male Wistar rats after. <strong>Materials and Methods:</strong> Experimental groups were administered with 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) followed by 5% Sodium ascorbate (Na-As), then they were post-treated with <em>Nigella sativa</em> (<em>N. sativa</em>) by an inter-gastric luminal gavage or with thymoquinone (TQ) by inter-peritoneal injections. Blood samples were collected after a 32 weeks’ experiment. <strong>Results</strong>: TQ seemed to have a powerful inhibitory effect on total cholesterol and triacylglycerol levels. While levels of A/G ratio, ALT and AST were highly decreased by both of <em>N. sativa</em> and TQ. <strong>Conclusion</strong>: These findings may rely on the lipid-modifying and hepatoprotective effects of <em>N.</em> <em>sativa</em> crude oil and its main constituent TQ on mammals. Thus, further investigations are required.