The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X3120190401Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice1123777110.21608/jcbr.2019.37771ENRanaMedhatBotany Department, Faculty of Science, Tanta University, EgyptMostafa M.El-SheekhDepartment of Botany, Faculty of ScienceAtef M.Abo-ShadyDepartment of Botany, Faculty of ScienceMohamed LSalemDepartment of Zoology, Immunology and Biotechnology Division, Zoology Department, Faculty of Science
Center of Excellence in Cancer Research Tanta University, Tanta, Egypt0000-0001-9454-6327Journal Article20180922<em>Spirulina platensis</em> (SP) is a blue-green alga used as a dietary supplement as it possesses antioxidant, anti-inflammatory and hepatoprotective effects with clinical importance in several disorders. Theaim of this study was to evaluate the ameliorating effects of SP extract on toxicity induced mice by treatment with the anticancer drug cyclophosphamide (CTX). To this end,SP was cultured under optimal conditions of growth at pH 9 for 9 days using Zarrouk<sup>,</sup>s medium. The active ingredients including pigments, phycobiliproteins and total soluble proteins were estimated in SPsuspensions which were then dried to a powder form. To assess anti-toxicity, adult male albino mice were treated with interperitoneal (i.p.) injection of PBS, single dose of CTX (4mg/mouse), CTX (i.p.) followed by subcutaneous (s.c.) injection of G-CSF (5μg/mouse) daily for 5 consecutive days or CTX (i.p.) followed by oral administration of 0.5 gm/mouse SP extract for 7 consecutive days. After 7 days of initial treatment with CTX, mice were sacrificed; blood, spleen, bone marrow and liver were harvested to assess CBC, total count for spleen and bone marrow and liver for biochemical analysis. The results showed that SP showed the highest yield of dry weight and pigment content C-phycocyanin (CPC), allophycocyanin (APC), phycoerthrin (PE) and total phycobiliproteins (phycobilins) at the 9<sup>th</sup> day of growth at pH 9 in culture. Oral administration of SP induced amelioration of CTX induced leucopenia in blood, bone marrow and spleen comparable to those of G-CSF. It also ameliorated the dysfunction in the liver enzymes ALT and AST. Interestingly, however, SP showed higher antioxidant effects than those of G-CSF as reflected by the higher activities of the anti-oxidant MDA and GSH. <strong>Conclusion:</strong>SP showed potent antitoxic effect through antioxidant activity and thus could be a useful co-adjuvant agent against chemotherapeutic drugs toxicity including cyclophosphamide.The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X3120190401Influence of Moringa oleifera flaxseed oil and atorvastatin on hyperlipidemic male albino rats13233777210.21608/jcbr.2019.37772ENSomia ZAbdelhalimProfessor of Physiology Zoology Department, Faculty of Science. Tanta University, EgyptMona M.HegaziZoology Department, Faculty of Science, Tanta University, Tanta, EgyptMohamed M.H.El-BagouryZoology Department, Faculty of Science, Tanta University, Tanta, EgyptJournal Article20190108The main aim of this study was to investigate the beneficial effects of lipitor (LIP), a commercial brand name of atorvastatin drug, flaxseed oil (FSO) and Moringa oleifera oil (MO), extract (ME) or meal (Mm) alone or in combination on hyperlipidemia induced in adult male albino rats. Rats were fed for 2 weeks on basal diet as a negative control group or fed on high fat diet (HFD) and a weekly dose of triton X-100 to induce hyperlipidemia as positive controls. The latter group was treated with: LIP, LIP + FSO, LIP + ME, FSO, FSO + ME, ME, MO or Mm for 4 weeks. At the end of the experiment, the livers were quickly excised and processed for analyzing the antioxidative enzymes activities including total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and malondialdehyde (MDA). Feeding on MO and ME) showed no effects on the body weight or on anti-oxidative enzyme activities. Feeding on FSO, however, induced only increase in the body weight. ME of dry leaves and roots showed a decrease in the body weight and improved antioxidative enzymes activities. These effects of FSO, ME and MO on the body weight of rats may be attributed to captivity and reduction of the energy expenditure, while their marked effects on the antioxidative enzyme activity may need longer time of treatments. These results indicate that Moringa oleifera can be used in packed food supplement products.The Egyptian Association for Cancer Research (EACR)International Journal of Cancer and Biomedical Research2682-261X3120190401Association between HCV infection and the incidence of Diabetes Mellitus in Egyptians24343776610.21608/jcbr.2019.37766ENYousry EEl-BolkinyDepartment of Zoology, Faculty of Science, Tanta University, Egypt0000-0003-4001-6034Fuad KHarrasInternal Medicine Department, Faculty of Medicine, Tanta University, Tanta, EgyptMerveet AMansourZoology Department, Faculty of Science, Tanta University, Tanta, EgyptAbdelhalim AAlkhazendarZoology Department, Faculty of Science, Tanta University, Tanta, EgyptMohamedRaghaeyInternal Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, EgyptJournal Article20180811The present work was planned to find a correlation between HCV infection and diabetes mellitus (DM) incidence in Egyptian patients by finding a possible relationship between HCV antibodies and both anti-insulin antibodies (AIAs) and pancreatic islet cell antibodies (PICAs). The screening results showed that the percentage of HCV patients out of 200 subjects were 35.5 and 14.5% in males and females, respectively. Also, the association between DM and HCV were 13.21 and 13.64% in men and women, respectively. The titers of antibodies in diabetic or non-diabetic patients ranged from 30 - 58 for AIAs and from 30 - 40 for PICAs, The percentage of patients with raised serum transaminases was significantly more in DM patients with positive HCV antibody (52.3%) than DM patients with negative HCV antibody (16.7%). Conclusion, it can be concluded that diabetic or non-diabetic patients with HCV might have a rise titer of the antibodies toward insulin or its secretory cells in pancreas or toward both. where are more susceptible to both HCV-infection and antibodies than women, indicating to here is a significant association between HCV infection and induction of diabetes in Egyptians.