Document Type : Original Article
Authors
1
Medical laboratory technology Department, Faculty of Allied Medical Sciences, Pharos University in Alexandria, Alexandria, Egypt.
2
Department of Biochemistry, Faculty of Science, Alexandria University, Egypt
3
Department of Food Technology, City for Scientific Research & Technology Applications, New Borg El Arab, Alexandria, Egypt.
4
Department of Medical Biotechnology, Genetic Engineering & amp; Biotechnology Research Institute, City for Scientific Research & Technology Applications, New Borg El Arab, Alexandria, Egypt.
5
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria Egypt.
Abstract
Background: Mycotoxins such as Fumonisin B (FB), are low-molecular-weight natural products produced as secondary metabolites by filamentous fungi. The mycotoxins, FB, contaminates grain products and acts as a virulence factor for host cells. Aim: This study aimed to evaluate the effect of ethanolic crude extracts of Cinnamuom zeilanicum (CZ), Berberise vulgaris (BV) and Calluna vulgaris (CV) on the growth of corn seed’s fungi and /or mycotoxin production. Materials and methods: Firstly, corn seeds were collected and cultured on water agar media and the grown fungi were inoculated into Sabouraud’s dextrose media, then the toxins were identified. Secondly, the dried plants of CZ, BV and CV were phytochemically screened for alkaloids, phlobatannins, saponnins, flavonoids, steroids, terpenoids and cardiac glycosides. The fungus purified DNA was sequenced, and BLAST alignment was used to identify the fungi and mycotoxin types. Furthermore, the effect of different plant extract treatments on fungi growth was assessed. Finally, toxinproduction was assessed using HPLC. Results: The obtained results confirmed that Fusarium isolate was Fusarium sporotrichioides. Among the three tested extracts, Cinnamuom zeilanicum extract was the most potent inhibitor for Fumonisin B production followed by Berberise vulgaris and Calluna vulgaris. Conclusion: Cinnamuom zeilanicum extract could be used during corn seed long-term packaging and storage to inhibit (FB) production and toxicity.
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