Adipose-derived mesenchymal stem cells ameliorate the damages induced by arsenic trioxide in the testis of rats

Document Type : Original Article


Department of Zoology, Faculty of Science, Assiut University, Assiut , Egypt


Background: Arsenic trioxide is an element present everywhere in the environment. Aim: The current investigation planned to explain the remedial impacts of adipose mesenchymal stem cells (AD-MSCs) of rats against harmful impacts of Arsenic trioxide (As2O3; As III) on the testis of rats. Materials and Methods: Sixty male rats were divided into six groups: Group (1): served as the control without any treatments. Group (2): received a daily oral dose of HCL (0.5 mg/kg b.w). Experimental groups (3 and 4) received a low dose (3mg/kg b.w) of As III and groups (5,6) received a high dose (15 mg/kg b.w) of As III. The rats were sacrificed after 15 days in groups (3,5). Groups (4,6) were injected with (0.6×106) AD-MSCs / 0.5 ml PBS immediately after 15 days of oral doses of As III in low and high dose then sacrificed 7 days after MSCs injection. Results: The results indicated that AD-MSCs decreased the levels of p53 and Bax and increased the level of Bcl2. Additionally, treatment with AD-MSCs rescued the toxicity of low and high doses (L &H) As III induced change in TNF-α, IL-6, and IL-10 levels. In addition, As III L &H + AD-MSCs administration resulted in the gradual elevation of CD44 mRNA compared to control untreated mice. low and high doses of As III prompted histopathological damages in testis were they mostly turned around by treatment of AD-MSCs. Conclusion: The present results demonstrate that AD-MSCs got from the adipose tissue of rats can be a powerful treatment against harmfulness induced by As III in testis.


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