Allam, N., Abd El-Zaher, E., allam, A. (2020). Immunological efficiency of microbial melanin on bacterial pathogenicity.. International Journal of Cancer and Biomedical Research, 4(3), 243-258. doi: 10.21608/jcbr.2020.35015.1051
Nanis Allam; Eman Abd El-Zaher; Asmaa allam. "Immunological efficiency of microbial melanin on bacterial pathogenicity.". International Journal of Cancer and Biomedical Research, 4, 3, 2020, 243-258. doi: 10.21608/jcbr.2020.35015.1051
Allam, N., Abd El-Zaher, E., allam, A. (2020). 'Immunological efficiency of microbial melanin on bacterial pathogenicity.', International Journal of Cancer and Biomedical Research, 4(3), pp. 243-258. doi: 10.21608/jcbr.2020.35015.1051
Allam, N., Abd El-Zaher, E., allam, A. Immunological efficiency of microbial melanin on bacterial pathogenicity.. International Journal of Cancer and Biomedical Research, 2020; 4(3): 243-258. doi: 10.21608/jcbr.2020.35015.1051
Immunological efficiency of microbial melanin on bacterial pathogenicity.
1Botany and Microbiology Department, Microbiology Unit,Faculty of Science, Tanta University.
2Botany and Microbiology Department ,Microbiology Unit (Mycology lab) , Faculty of science, Tanta Univercsity.
3Botany and Microbiology department, tanta university
Abstract
In our present study, the immunological efficiency of brown Mel1 extracted from Streptomyces longisporoflavus NR043926 and black Mel2 extracted from Aspergillus niger MT355517 on bacterial pathogenicity was investigated. Mel1 and Mel2 were extracted and purified from the dried microbial biomass. Chemical characterization of extracted MeI pigments were compared with standard melanin. In vivo, estimation of the protective action of these pigments against Escherichia coli infection in Swiss albino mice which was identified by molecular characterizations 16S rRNA gene sequences, Albumin content was reduced in +ve control group (infected non – treated mice), while liver enzymes (ALT & AST) were increased in comparison to normal values were detected in immunized mice with Mel pigments. Eosinophil, monocyte values showed a non-difference, while the changes in lymphocyte numbers were paralleled to the response of total leukocyte counts. Serum antibodies total IgM in the non-immunized (+ve control group) mice were markedly lower than those observed in immunized mice (prevention groups and treated groups) with microbial melanin which can stimulate immunity system. Mel2 had high immunoprotection defense against E. coli infection in comparison to Mel1.
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