Ziada, M., Mona, M., Basyony, M., Salem, M. (2021). In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis. International Journal of Cancer and Biomedical Research, 5(1), 155-164. doi: 10.21608/jcbr.2021.54156.1105
Mai Ziada; Mohamed Mona; Mohamed Basyony; Mohamed Salem. "In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis". International Journal of Cancer and Biomedical Research, 5, 1, 2021, 155-164. doi: 10.21608/jcbr.2021.54156.1105
Ziada, M., Mona, M., Basyony, M., Salem, M. (2021). 'In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis', International Journal of Cancer and Biomedical Research, 5(1), pp. 155-164. doi: 10.21608/jcbr.2021.54156.1105
Ziada, M., Mona, M., Basyony, M., Salem, M. In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis. International Journal of Cancer and Biomedical Research, 2021; 5(1): 155-164. doi: 10.21608/jcbr.2021.54156.1105
In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
Abstract
Background: Natural products, those extracted from invertebrate animals have medical importance as therapeutic agents especially for cancer treatment. Aim of the Work: The current study was conducted to evaluate the antitumor effect of the hemolymph of the desert snail, Helix desertorum (HD-H) on tumor Ehrlich ascetic carcinoma cells (EAC) cell line in vitro and in vivo. Materials and Methods: Helix desertorum (HD-H) was collected from their suitable habitats, identified, and then their hemolymph was collected, to evaluate their antitumor effect. 40 female albino mice were divided into five groups (n=8) as the following: group 1 (Gp1) control mice were inoculated intraperitoneal (i.p) with 1x106 EAC cells/mouseat day 0, Gp2 (EAC/Cis): Mice were injected with EAC-cells as in Gp1 and at day 2 were injected with cisplatin (40 mg/Kg), Gp3 was inoculated with EAC and then treated with splenocytes activated with HD-H, Gp4was inoculated with EAC and then treated with splenocytes activated with IL-2/Con A, and Gp5 was inoculated with EAC and then treated with splenocytes without activation. All groups were sacrificed to evaluate the tumor profile, hematological and biochemical. Results: The results showed that treatment with HD-H led to decrease tumor volume, their cell counts, increase the percentage of the apoptotic cells and arresting the cancer cell cycle. Moreover, treatment with HD-H improved the hematological and biochemical parameters on tumor-bearing mice. Conclusion: In conclusion, HD-H has potential in vitro and in vivo antitumor effects against EAC-cells. Further study is recommended to evaluate the potential efficacy of HD-H as a potential anticancer
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