Hussein, N., Ebied, S., Saleh, M. (2021). Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells. International Journal of Cancer and Biomedical Research, 5(2), 81-97. doi: 10.21608/jcbr.2021.46268.1079
Neveen A. Hussein; Samia Ebied; Marwa Saleh. "Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells". International Journal of Cancer and Biomedical Research, 5, 2, 2021, 81-97. doi: 10.21608/jcbr.2021.46268.1079
Hussein, N., Ebied, S., Saleh, M. (2021). 'Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells', International Journal of Cancer and Biomedical Research, 5(2), pp. 81-97. doi: 10.21608/jcbr.2021.46268.1079
Hussein, N., Ebied, S., Saleh, M. Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells. International Journal of Cancer and Biomedical Research, 2021; 5(2): 81-97. doi: 10.21608/jcbr.2021.46268.1079
Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells
1Applied Medical Chemistry, Medical Research Institute, Alexandria, Egypt.
2Medical Research Institute, Alexandria, Egypt.
Abstract
Background: Cancer researchers have been concentrated on finding the best therapeutic strategies to decline cancer mortality rates. Marine natural products with pharmacological activities have potent anticancer activities. Aim: This study investigated the anticancer effect of violacein, phycocyanin, and phycocyanobilin in MCF-7 cells. Additionally, the elucidation of combined therapy efficiency (violacein-phycocyanin) on enhancing the anticancer activity of monotherapy. Materials and Methods: The cultured untreated, and treated cells by etoposide, violacein, phycocyanin, phycocyanobilin, and combined therapy were subjected to molecular studies, and glycan profiles by real time-PCR, and MALDI-TOF respectively. Results: Bax, Bcl-2, caspase-3 genes expression, and Bax/Bcl-2 ratio in all groups were significantly upregulated compared to the untreated cells except for a significant decrease in Bcl-2 by etoposide or combined therapy and an insignificant difference in Bax/Bcl-2 by PC or PCB treatment. The intensity of tetra-, tri-sialylated N-glycan, and di-sialylated O-glycan was significantly decreased in violacein, and combined-treated cells when compared to untreated or PC-treated cells. Mono-sialylated O-glycan was also decreased in combined therapy compared to the treated or untreated cells. Conclusion: Cotreatment with violacein/PC generated synergistic, antiproliferative, and pro-apoptotic effects on MCF-7 cells. Therefore, these compounds may be promising chemotherapeutic agents for breast cancer
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