Detection of Circulating Cancer Stem Cell and Matrix Metalloproteinase-2 in Patients with Hepatitis C Virus and their Correlation with Hepatocellular Carcinoma: Thesis Abstract

Document Type : Essay Abstract

Author

Immunology Department, Medical Research Institute, Alexandria University, Egypt

Abstract

Thesis Abstract
Hepatitis C virus (HCV) infection is a major health problem. In majority of infected patients, HCV can effectively evade innate immunity resulting in chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Similar to most tumors, HCC contain poorly differentiated cancer stem cells (CSCs) that initiate tumorigenesis. The interaction of stem cell with microenvironment is key regulatory mechanism in maintenance of its self-renewal capacities. Matrix metalloproteinase (MMPs) are proteolytic enzymes that plays a role in degradation of extracellular matrix which is necessary for metastasis. The present work attempted to study the mRNA expression of CSC markers (CD133 and CD44) as well as levels of MMP-2 in HCV patients and their correlation with progression towards cirrhosis and HCC. Peripheral blood mononuclear cell (PBMC) prepared from HCV patients (either with or without complications) were probed for mRNA expression of CD133 and CD44 by RT-PCR and compared to that of non-HCV cirrhotic patients as well as healthy controls. Also MMP-2 levels were measured using ELISA technique. Our results revealed that mRNA expression of CD133 was significantly elevated in HCV patients without complications while maximal CD44 mRNA expression and maximal circulating MMP-2 levels was noticed in HCC patients. Taken together, these results suggests that, chronic HCV infection appear to predispose cells towards path of acquiring cancer stem cell traits by inducing CD133 and CD44 expression leads to their transformation into CSCs. In addition HCV has important role in creating a microenvironmental change by enhancing MMP-2 release that increase invasion potential and facilitate tumor progression.

Keywords

Main Subjects

References

Manal A. Nabil1,*
Laila H. El-Sayed1
Hossam M. Ghoneim1
Saeid A. Oda2
Abdel Rahman M
A3 1Departement of Immunology & Allergy, Medical Research Institute, Alexandria University, Egypt. 2Departement of Internal Medicine, Medical Research Institute, Alexandria University, Egypt. 3Departementof Clinical Pathology, Alexandria Military
Egyptian Journal of Cancer and Biomedical Research
Volume 5, Issue 1.1 - Serial Number 1
Special Issue: Thesis Abstracts
May 2021
Pages 11-11

Files

Share

How to cite

Statistics