Document Type : Original Article
Authors
1
Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
2
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
3
Chemistry Department, Faculty of Science, Tanta University, Egypt. Center of Excellence in Cancer Research.
4
Chemistry Department, Faculty of Science, Tanta University.
Abstract
Background: Polymeric nanoparticles (NPs) are obtained naturally, semi-synthetically or synthetically by a polymerization reaction. Tetronics based ethylene oxide-propylene oxide copolymers have gained a great of interest. It demonstrated interactions with cell membranes with potential for developing new biomaterials for application in nanomedicine . Aim: The primary aim of the current study is to test the direct anticancer activity of unmodified tetronic (T1107), P-HB+aminated tetronic and N, N DMAB+aminated tetronic polymersand the associated by apoptosis and cell cycle. Materials and Methods: Synthetic polymers were prepared and characterized to confirm the modification. Anti-tumor activity was examined in vitro using human hepatocellular carcinoma (HepG2) cell line. Cell viability (MTT), cell cycle and apoptosis were evaluated by flow cytometry. Results: Unmodified tetronic (T1107) decreased HepG2 viability (by 30%) than untreated HepG2 cells. Treatment with P-HB+aminated tetronic and N,N DMAB+aminated did not give a significant effect on Hepg-2 cells. Treatment of HepG2 with unmodified tetronic (T1107) induced cell cycle arrest at G0 phase (39.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic induced cell cycle arrest at G1 phase (by 54.8% and 48.2%, respectively as compared to treatment with the doxorubicin (DOX) as a reference drug, which induced Hepg-2 cell cycle arrest at GO phase (by 39.1%). The unmodified tetronic (T1107) increased the numbers of late apoptotic cells (by 49.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic did not induced significant apoptosis. Conclusion:.Unmodified Tetronic T1107 induces an anticancer effect more than modified Tetronic polymers.
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