Chemotherapy upregulates gene expression of IL-1 and prostaglandin in the blood of patients with non-Hodgkin’s lymphoma

Khalil, Sohaila M.; Samy, Doaa; El-atrash, Afaf Mohktar; Ageba, Mohamed Fouad; Salem, Mohamed Labib

doi:10.21608/jcbr.2022.116128.1245

Chemotherapy upregulates gene expression of IL-1 and prostaglandin in the blood of patients with non-Hodgkin’s lymphoma

Document Type : Original Article

Authors

¹ zoology department, faculty of science, tanta university, egypt

² zoology department, faculty of science, Tanta university

³ Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt

⁴ Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

10.21608/jcbr.2022.116128.1245

Abstract

Background: Most lymphoma patients, including non-Hodgkin lymphoma (NHL), show dysfunction in immunity, especially after chemotherapy, resulting in a lack of responses to treatment and tumor relapses. This dysfunction could be due to the high expression of inflammatory mediators, especially prostaglandin, including its different types and critical cytokines such as IL-1. Aim: This pilot study aimed to measure gene profiling of IL-1 and PG pathways in the peripheral blood of NHL patients. This study was conducted on 3 healthy volunteers and 9 NHL patients; before, during, and after induction of chemotherapy CHOP (chemotherapy regimen consists of Cyclophosphamide, Hydroxydaunorubicin, Oncovin and Prednisone). The patients were recruited from Tanta Cancer Centre, Tanta. Total RNA (mRNA) was extracted, and then IL-1 (NFKB-1, PELI-1, IL-1α, CCL-2, and CASP-1), Prostaglandin (PTGS-2, PTGER-2, and EDN-1) pathways were analyzed by Gene Chip RNA HTA 2.0 Arrays (Affymetrix). Real-time PCR (qRT-PCR) was used as validation of the genes of the IL-1 and PG pathways. Results: Microarray analysis showed that gene expression of IL-1 pathways, including NFKB1, PELI-1 and CCL2, was downregulated at early diagnosis as compared to healthy control; their expression was upregulated during as well as after chemotherapy. CASP-1 and IL-1α gene expression, however, was downregulated in the early-diagnosed group and then upregulated after chemotherapy. PTGS-2, PTGER2 and EDN1 in the prostaglandin pathway were downregulated in early-diagnosed patients and then, upregulated during chemotherapy as well as after chemotherapy. Conclusion: Chemotherapy regulates the expression of certain genes of the IL-1 and Prostaglandin pathways. These inflammatory mediators may be represented as biomarkers in diagnoses and prognosis

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