Adipose mesenchymal stem cell-based therapy: Anticancer molecular mechanisms in hepatoma model and bone marrow

Atia, Mona M.; Sallam, Ebtehal T.; Aly, Hanem S.; Alghriany, Alshaimaa A. I.

doi:10.21608/jcbr.2022.163847.1278

Adipose mesenchymal stem cell-based therapy: Anticancer molecular mechanisms in hepatoma model and bone marrow

Document Type : Original Article

Authors

¹ Department of Zoology and Entomology, Faculty of Science, Assiut University, 71516, Egypt

² Department of Biology, Faculty of Education, Taiz University, Yemen.

³ Department of Zoology and Entomology , Faculty of Science, Assiut University, 71516, Egypt

10.21608/jcbr.2022.163847.1278

Abstract

ABSTRACT

Background: Adipose-derived mesenchymal stem cells (AD-MSCs) can be used as therapeutic agents for the treatment of patients with HCC Aim: The study aimed to establish an animal model for hepatocellular carcinoma (HCC) by using diethyl nitrosamine (DEN) and carbon tetrachloride (CCl4) in a short time. Materials and Methods: Forty-five female rats were subdivided into three groups: control (G1), HCC model (G2), and therapeutic (G3). Each rat in the G2 groups was injected with DEN and after 1 week was injected again with CCl4, whereas G3 rats were injected with AD-MSCs immediately after the tumor appeared. Results: The injection of DEN/CCl4 was correlated with the increase in alanine transaminase, aspartate aminotransferase, and alpha-fetoprotein levels. Also, it caused oxidative stress as indicated by an increase in nuclear factor-erythroid factor 2-related factor-2 (Nrf2), vascular endothelial growth factor levels, and fibrosis. However, BCL2-associated X protein (BAX) was decreased. Transplantation of AD-MSCs induced apoptosis by increasing BAX and decreasing Nrf2 levels, which significantly inhibited angiogenesis. Histologically, our results indicated that AD-MSCs alleviated hepatocellular carcinoma, fibrosis, and nuclear morphology. Additionally, the bone marrow (BM) maturation index ratio parameter was restored to a normal stage. Conclusion: AD-MSCs have a wide range of targeted anticarcinogenic properties and can regenerate BM precursors of cellularity.

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