RETN gene polymorphisms and serum resistin levels in patients with breast cancer

Document Type : Original Article

Authors

1 Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt

2 Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Tanta University, Egypt

3 Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt

Abstract

Background: Increased serum resisitin levels act as an effective pro-inflammatory mediator, which may play a key role in cancer progression and promotion and may be caused by RETN gene polymorphisms. Aim of the work: We investigated serum resistin levels in breast cancer patients as well as the relationship between the RETN single nucleotide polymorphisms (rs7408174 and rs3219175) and breast cancer susceptibility. Subjects and Methods: This case control study included 80 breast cancer patients and 80 healthy controls. Genotyping of RETN rs7408174 and rs3219175 gene polymorphism were determined by real time polymerase chain reaction (RT-PCR) TaqMan allelic discrimination assay. Serum resistin levels were quantified using Enzyme-linked immunosorbent assay (ELISA). Results: Breast cancer patients had higher serum resistin levels compared to healthy controls. Patients with rs3219175 AG and rs7408174 CT/CC genotypes had significantly higher serum levels of resistin. Carriers of the single nucleotide polymorphism (SNP) rs3219175 heterozygous (AG) and homozygous (AA) genotypes had a significantly higher breast cancer risk than carriers of the wild (GG) genotype (Odd ratio (OR): 2.437; 95% CI: 1.262-4.707 and 3.091; 0.723-13.222, respectively). Patients carrying rs3219175 AG/AG+AA and rs7408174 CT/CC genotypes had a higher risk of developing advanced tumour size (T3+T4), lymph node (LN) infiltration, and advanced TNM stage (III+IV). Conclusion: There is a strong link between RETN SNP rs3219175 and high serum resistin levels and the risk of breast cancer. Furthermore, the RETN SNPs rs3219175 and rs7408174 are linked to poor clinicopathological status.

Keywords

Main Subjects

Egyptian Journal of Cancer and Biomedical Research
Volume 8, Issue 4
December 2024
Pages 1-10

Files

Share

How to cite

Statistics