Document Type : Original Article
Authors
Department of Zoology, Science Faculty, University of Tanta, Tanta, Egypt
10.21608/jcbr.2025.425699.1420
Abstract
This research evaluated the prophylactic immunomodulatory and anti-inflammatory potentials of silymarin (Sylm), curcumin (Curc), amygdalin (Amyg), and ginger (Ging) on carbon tetrachloride (CCL4)-induced liver inflammation. Mice were divided into six groups: group 1 received saline; group 2 received STZ (40 mg/kg daily) for 5 days followed by normal saline; and groups 3 to 6 received herbal extracts: Sylm (112 mg/kg), Curc (522 mg/kg), Amyg (64 mg/kg), and Ging (115 mg/kg), respectively, thrice/week for 3 weeks. Then, they were given STZ (40 mg/kg daily) for 5 days; 24 h following each CCL4 injection, they were given again herbal extracts at the same doses twice a week for 6 weeks. The expression of natural killer (NK) cells, CD4+ T cells, and CD8+ T cells; splenocyte necrosis rates; pro-inflammatory markers necrosis factor-α (TNF-α) and alpha fetoprotein (AFP); and total and differential leukocyte counts, along with liver and kidney functions, were assessed. As a result, mice treated with herbal extracts from Sylm, Curc, Amyg, or Ging post-CCL4 injection exhibited significant increases in phenotypic expressions of NK, CD4+, and CD8+ T cells, while TNF-α, AFP levels, and splenocyte necrosis rates were significantly reduced compared to CCL4-intoxicated controls. Moreover, Amyg is regarded as the most effective extract for regulating immune responses in liver inflammation. To conclude, herbal therapies from Sylm, Curc, Amyg, and Ging show promise in regulating immune responses to chemical damage, highlighting their potential in treating liver inflammation. Further clinical trials are essential for proving their immune regulation and therapeutic effectiveness in liver inflammation.
Keywords
Main Subjects
)
View on SCiNiTO