Evaluation of the anti-tumor and immunomodulatory effects of synthetic chalcones in a tumor mouse model

Document Type : Original Article

Authors

1 Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

2 Department of Chemistry, Faculty of Science, Tanta University, Egypt

Abstract

Introduction: Traditional treatment of cancer by using chemotherapy not only kills cancer cells but also normal cells. It also fails in the complete prevention of the spread of tumor cells. Chalcone have several biological activities, such as antioxidant, antibacterial, antifungal, anti-inflammatory, and anti-tumor. Aim: To compare the antitumor effects of chalcones in Ehrlich ascetic carcinoma (EAC) bearing mice. Materials and Method: Female Swiss albino mice were randomly divided into 6 groups as the following; Group 1 were served as a negative control, group 2 were inoculated with 2.5×105 (EAC) cells, group 3 were inoculated with 2.5×105 EAC cells and then treated with cisplatin (2 mg/kg), group 4 were inoculated with 2.5×105 EAC cells and then treated with chalcone 1 (0.3 ml gm/L), group 5 were inoculated with 2.5×105 EAC cells and then treated with chalcone II (0.3 ml gm/L). After 7 days, all groups of mice were sacrificed to measure the number and cell cycle of tumor cells as well as the number and activation of CD8+ T cells. Results: Treatment of tumor-bearing mice with chalcone I, but not chalcone II induced decreases in the total number of live cells when compared to control tumor-bearing mice, coinciding with significant increases in G0, G1, S and G2 phases of EAC cells which were comparable to the effects of cisplatin. As compared to control tumor-bearing mice, tumor cells harvested form mice treated with chalcone I showed significant increases in the numbers of activated CD8+ T cells. Conclusion: Chalcone I possesses anti-tumor effects by inducing tumor cells arrest and activation of T cells.

Keywords

Main Subjects

Files

Share

How to cite

Statistics